Coding

Part:BBa_K1112002:Design

Designed by: Alba Iglesias Vilches   Group: iGEM13_Valencia_Biocampus   (2013-09-10)


Cluster PHA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1819
    Illegal PstI site found at 718
    Illegal PstI site found at 2946
    Illegal PstI site found at 4335
    Illegal PstI site found at 4755
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1819
    Illegal PstI site found at 718
    Illegal PstI site found at 2946
    Illegal PstI site found at 4335
    Illegal PstI site found at 4755
    Illegal NotI site found at 3106
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1819
    Illegal XhoI site found at 2271
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1819
    Illegal PstI site found at 718
    Illegal PstI site found at 2946
    Illegal PstI site found at 4335
    Illegal PstI site found at 4755
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1819
    Illegal PstI site found at 718
    Illegal PstI site found at 2946
    Illegal PstI site found at 4335
    Illegal PstI site found at 4755
    Illegal NgoMIV site found at 90
    Illegal NgoMIV site found at 1620
    Illegal NgoMIV site found at 1747
    Illegal NgoMIV site found at 1996
    Illegal NgoMIV site found at 2047
    Illegal NgoMIV site found at 3915
    Illegal NgoMIV site found at 4368
    Illegal AgeI site found at 374
    Illegal AgeI site found at 1894
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PHA, or polyhydroxyaalkanoates, is a bioplastic produced in nature by bacterial fermentation of sugar or lipids. In our case it is produced by the fermentation of octanoic acid.


Source

It is a naturally present sequence in Pseudomonas. Allows bioplastic (PHA) synthesis. PHA, or polyhydroxyaalkanoates, is a bioplastic produced in nature by bacterial fermentation of sugar or lipids. In our case it is produced by the fermentation of octanoic acid.

References

1. Wang HH, Zhou XR, Liu Q, Chen GQ. Biosynthesis of polyhydroxyalkanoate homopolymers by Pseudomonas putida. Appl Microbiol Biotechnol. 2011 Mar;89(5):1497-507. doi: 10.1007/s00253-010-2964-x. Epub 2010 Nov 3. 2. Nelson KE et al. Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440. Environ Microbiol. 2002 Dec;4(12):799-808.